WHO program «MONICA-psychosocial»: insomnia and biological markers in the male population aged 25-64 years

Objective: to study the association polimorfizma-G308A gene tumor necrosis factor TNF-α and sleep disturbances in the male population aged 25-64 in metropolis of Western Siberia (Novosibirsk). Methods: Under the III screening of the WHO program «MONICA-psychosocial» a representative sample of men 25-64 years were surveyed (n = 657 men, mean age - 44, 3 ± 0,4 years responsum - 82.1%) in 1994 in Novosibirsk. For assessment of sleep levels scale Jenkins was used. Procedures have been standardized and strictly comply with the requirements of the protocol of the «MONICA». Genotyping of polymorphisms studied gene-G308A tumor necrosis factor TNF-α was conducted in the laboratory of molecular genetic in Research Internal Medicine and Preventive Medicine. Statistical analysis was performed using the software package «SPSS-11,5». Results: The level of sleep disorders in the male population aged 25-64 was 48.3%: evaluation of sleep as «fair» was 39.6%, «bad» - 7.6%, «very bad» - 1.1%. Genotype G/G gene TNF-α was in 79.1% of individuals, the genotype A/G - 19% of cases and genotype A/A was in 1.9% of men. Men carrying the genotype G/G of TNF-α gene are more likely to assess their sleep as «good» (98.3%), compared to those carrying other genotypes. In contrast subjects with heterozygous genotype A/G of TNF-α gene are more likely to estimate their sleep as «fair» (30%) than «good» (15.2%) in all groups. Conclusion: It was determined a heterozygous genotype A/G of TNF-a gene is most frequently associated with sleep disorders.

инсомния, открытая популяция, биологические маркеры, insomnia, an open population, biological markers

1. Александровский Ю.А., Аведисова А.С. Инсомния и некоторые особенности действия современных гипнотиков // Клиническая фармакология. 1995. № 4. C. 80-85.

2. Бююль А., Цёфель П. [Buul A., Cofel P.]. SPSS: искусство обработки информации. Анализ статистических данных и восстановление скрытых закономерностей: Пер. с нем. СПб.: ООО DiaSoftЮП 2002. 608 c.

3. Allen R.A, Lee E.M, Roberts D.H, Park B.K, Pirmohamed M. Polymorphisms in the TNF-alpha and TNF-receptor genes in patients with coronary artery disease // Eur J Clin Invest. 2001. Vol. 31. P. 843-51.

4. Beneto A. et al. Comorbidity between sleep apnea and insomnia // Sleep Med. Rev. 2009. Vol. 13. P. 287-293.

5. Centers for Disease Control and Prevention (CDC) Effect of short sleep duration on daily activities - United States, 2005-2008 // MMWR Morb Mortal Wkly Rep. 2011. Vol. 60. P. 239-52.

6. Colten H.R., Altevogt B.M. Sleep disorders and sleep deprivation: an unmet public health problem. Washington, DC: National Academies Press, 2006. 424 p.

7. Dalziel B., Gosby A.K., Richman R.M., Bryson J.M., Caterson I.D. Association of the TNF-alpha -308 G/A promoter polymorphism with insulin resistance in obesity // Obes Res. 2002. № 10. P. 401-7.

8. Glants С. Biomedical statistics. М: Practika, 1998. 459 p.

9. Huang J., Liao N., Huang Q.P., Xie Z.F. Association between tumor necrosis factor-a-308G/A polymorphism and obstructive sleep apnea: a meta-analysis // Genet Test Mol Biomarkers. 2012. Vol. 16(4). P. 246-51.

10. Jeanmonod P., von Kanel R., Maly F.E., Fischer J.E. Elevated Plasma C-reactive protein in chronically distressed subjects who carry the A allele of the TNF-alpha -308 G/A polymorphism // Psychosom Med. 2004. Vol. 66. P. 501-6.

11. Jun T.Y., Pae C.U., Hoon H., Chae J.H., Bahk W.M., Kim K.S., Serretti A. Possible association between -G308A tumour necrosis factor-alpha gene polymorphism and major depressive disorder in the Korean population. Psychiatr Genet. 2003. V. 13. P. 179-81.

12. Nicaud V., Raoux S., Poirier O., Cambien F., O'Reilly D.S., Tiret L. The TNF alpha/G-308A polymorphism influences insulin sensitivity in offspring of patients with coronary heart disease: The European Atherosclerosis Research Study II // Atherosclerosis. 2002. Vol. 161. P. 317-25.

13. Tomasdottir H., Hjartarson H., Ricksten A., Wasslavik C., Bengtsson A., Ricksten S.E. Tumor necrosis factor gene polymorphism is associated with enhanced systemic inflammatory response and increased cardiopulmonary morbidity after cardiac surgery // Anesth Analg. 2003. Vol. 97. - P. 944-9.

14. Vendrell J., Fernandez-Real J.M., Gutierrez C., Zamora A., Simon I., Bardaji A., Ricart W., Richart C. A polymorphism in the promoter of the tumor necrosis factor-alpha gene (-308) is associated with coronary heart disease in type 2 diabetic patients // Atherosclerosis. 2003. Vol. 167. P. 257-64.

15. Warzocha K., Ribeiro P., Jacques Bienvenu, Pascal Roy, Carole Charlot, Dominique Rigal, Bertrand Coiffier, Salles G. Genetic polymorphisms in the Tumor Necrosis Factor Locus Influence Non-Hodgkin’s Lymphoma Outcome // Blood. 1998. Vol. 91(10). P. 3574-3581.

16. Weinberger J.F., Raison C.L., Rye D.B., Montague A.R., Woolwine B.J., Felger J.C., Haroon E., Miller A.H. Inhibition of tumor necrosis factor improves sleep continuity in patients with treatment resistant depression and high inflammation // Brain Behav Immun. 2015. Vol. 47. P. 193-200.

17. Wilson A.G., Symons J.A., McDowell T.L., McDevitt H.O., Duff G.W. Effects of a polymorphism in the human tumor necrosis factor alpha promoter on transcriptional activation // Proc Natl Acad Sci USA. 1997. Vol. 94. P. 3195-9.

18. WHO MONICA Project prepared by Kuulasmaa K. et al. Baseline population survey data book. MONICA Memo 178 A. Helsinki: [s.n.], 1990. 39 p.

19. WHO Proposal for the Multinational Monitoring of Trends in cardiovascular disease. Geneva: [s.n.], 1985. 67 p.

20. World Health Organization. MONICA Psychosocial Optional Study. Suggested Measurement Instruments. Copenhagen: WHO Regional Office for Europe, 1988. 33 p.

21. Zielinski M.R, Krueger J.M. Sleep and innate immunity // Front Biosci (Schol Ed). 2011. Vol. 3. P. 632-642.